determination of vitamin c in small volumes of blood by hplc/ec

نویسندگان

abbas hadjiakhoondi

department of pharmacognosy, faculty of pharmacy, tehran university of medical sciences, tehran, iran david g. watson

department of pharmaceutical sciences, strathclyde institute for biomedical sciences, 27 taylor st., glasgow g4 0nr, u.k. samad lotfollahzadeh

department of clinical sciences, faculty of veterinary medicine, tehran university, tehran, iran mohammad reza mokhber dezfouli

department of clinical sciences, faculty of veterinary medicine, tehran university, tehran, iran

چکیده

a sensitive procedure for determining total vitamin c (ascorbic acid + dehydrate ascorbic acid) in a blood drop from a finger prick, before and after the adminis- tration of a vitamin c tablet is described. analysis was carried out by high per- formance liquid  chromatography with  electrochemical detection (hplc/ec). measurements were taken one hour, two hours and six hours after the administra- tion of a 500 mg vitamin c tablet. d-isoascorbic acid was used as the internal standard and analysis was carried out using two c-18 columns connected in series and a phosphate buffer mobile phase. dehydroascorbic acid in the samples was converted to ascorbic acid by incubation with dl-homocysteine for 30 minutes. the level of vitamin c in blood reached a maximum concentration after two hours.

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Determination of Vitamin C in Small Volumes of Blood by HPLC/EC

      A sensitive procedure for determining total vitamin C (ascorbic acid + dehydrate ascorbic Acid) in a blood drop from a finger prick, before and after the adminis- tration of a vitamin C tablet is described. Analysis was carried out by high per- formance liquid  chromatography with  electrochemical detection (HPLC/EC). Measurements were taken one hour, two hours and six hours after the adm...

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عنوان ژورنال:
iranian journal of pharmaceutical sciences

جلد ۱، شماره ۱، صفحات ۷-۱۰

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